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Academic Journal of Second Military Medical University ; 41(3):307-308, 2020.
Article Dans Chinois | GIM | ID: covidwho-833130

Résumé

Coronavirus disease 2019 (COVID-19) is highly infectious, and the risk of cross infection is high among front-line medical staff. In our department, we have made full use of bed call intercom system and monitoring system in the isolation wards for COVID-19, and also tried to use mobile phones, walkie talkie and other means of communication. These means greatly reduced occupational exposure, close contact between medical staff and patients, nosocomial infection risk and the use of medical protective equipment, which improves the efficiency of diagnosis and treatment.

2.
Academic Journal of Second Military Medical University ; 41(4):365-370, 2020.
Article Dans Chinois | GIM | ID: covidwho-829836

Résumé

Objective To isolate the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from nasal/throat swabs of coronavirus disease 19 (COVID-19) patients. Methods Three nasal/throat swab samples from COVID-19 patients in Shanghai were treated with TPCK trypsin and were used to treat Vero E6 cells inoculated in 96-well plates. When most of the cells showed obvious cytopathy, the cell culture supernatants were collected. We then detected the viral nucleic acid by fluorescent quantitative real-time polymerase chain reaction, and amplified the gene fragment of the virus receptor binding domain (RBD) by reverse transcription polymerase chain reaction. After amplification and culture, the virus was used to infect the Vero E6 cells inoculated in 96-well plates. The cytopathy was observed and the virus protein was detected by immunofluorescence. Results The Vero E6 cells that cultured with two of three nasal/pharyngeal swab samples showed obvious cytopathic effect and newly synthesized viral nucleic acid was detected in the supernatants of the cell culture. The amplified RBD sequence was completely consistent with the corresponding fragment of SARS-CoV-2 isolated earlier. Virus-infected Vero E6 cells showed cytopathies rapidly and could react with the monoclonal antibody against nucleocapsid protein (N protein) and spike protein (S protein) of SARS-CoV-2, and convalescence sera of COVID-19 patients. Conclusion Two SARS-CoV-2 strains were successfully isolated from two nasal/throat swab samples of COVID-19 patients in Shanghai, which provides evidence for the mechanism research on the infection and pathogenesis of SARS-CoV-2 as well as the development of drugs and vaccines against SARS-CoV-2.

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